Preparation of a homogeneous soluble D-beta-hydroxybutyrate apodehydrogenase from mitochondria.

Photolabelling of D-beta-hydroxybutyrate apodehydrogenase with azidoaryl phospholipids.

Two synthetic photoactive azidoarylphosphatidylcholines were used to investigate the level of interaction between D-beta-hydroxybutyrate apodehydrogenase (apoBDH), an amphipathic membrane protein, with the hydrophobic domain of phospholipids. The two synthetic lecithins, PL I (1-myristoyl-2-12-N-(4-azido-2-nitrophenyl) aminododecanoyl-sn-glycero-3-phosphocholine) and PL II (1-myristoyl-2-(2-azi...

The structural specificity of lecithin for activation of purified D-beta-hydroxybutyrate apodehydrogenase.

n-/3-Hydroxybutyrate dehydrogenase is a lipid-requiring enzyme which has an absolute requirement for lecithin for enzymic activity. We have studied the activation of the purified apodehydrogenase by a number of lecithin analogues with modifications in either the hydrophobic or polar regions of the molecule in order to map the structural specificity for the lecithin molecule. The apodehydrogenas...

Characterization of two D-beta-hydroxybutyrate dehydrogenase populations in heavy and light mitochondria from jerboa (Jaculus orientalis) liver.

Mitochondrial membrane-bound and phospholipid-dependent D-beta-hydroxybutyrate dehydrogenase (BDH) (EC 1.1.1.30), a ketone body converting enzyme in mitochondria, has been studied in two populations of mitochondria (heavy and light) of jerboa (Jaculus orientalis) liver. The results reveal significant differences between the BDH of the two mitochondrial populations in terms of protein expression...

D-beta-hydroxybutyrate extends lifespan in C. elegans

The ketone body beta-hydroxybutyrate (βHB) is a histone deacetylase (HDAC) inhibitor and has been shown to be protective in many disease models, but its effects on aging are not well studied. Therefore we determined the effect of βHB supplementation on the lifespan ofC. elegans nematodes. βHB supplementation extended mean lifespan by approximately 20%. RNAi knockdown of HDACs hda-2 or hda-3 als...

Preparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures